This electrophoretic migration based extracellular vesicle (EV) isolation system achieved high size selective isolation in blood plasma as ultracentrifuge do.
EV isolated with this system has 8 times more EV amount than ultracentrifuge according to RNA level.
As different to the precipitation method, isolated EV with this system did not have chemical artifact hindering post-processes: RT-PCR, western-blot and ELISA.
Only half of residual protein remain with this system compared to the precipitation method.