Novel chitin/chitosan-glucan wound dressing: Isolation, characterization, antibacterial activity and wound healing properties

详细信息    查看全文

• 作者：A.M. Abdel-Mohsen^a ; ^b ; ^c ; ^{abdel-mohsen@ceitec.vutbr.cz" class="auth_mail" title="E-mail the corresponding author} ; ^{abdo_mohsennrc@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; J. Jancar^a ; ^b ; ^d ; D. Massoud^e ; Z. Fohlerova^a ; H. Elhadidy^f ; ^g ; Z. Spotz^a ; A. Hebeish^c
• 关键词：ChGC ; chitin-glucan complex ; ChCsGC ; chitin/chitosan-glucan complex ; TGA ; thermal gravimetric analysis ; SEM ; scanning electron microscopy ; TEM ; transmission electron microscopy ; DSC ; differential scanning calorimetry ; IPA ; iso-propyl alcohol ; S. commune ; Schizophyllum commune ; NMR ; nuclear magnetic resonance ; S/L ; solid to liquid ratio ; FTIR-ATR ; Fourier transform infrared spectroscopy ; XRD ; X-ray diffraction ; E. coli ; Escherichia coli ; K. pneumoniae ; Klebsiella pneumoniae ; B. subtilis ; Basillus
• 刊名：International Journal of Pharmaceutics
• 出版年：2016
• 出版时间：20 August 2016
• 年：2016
• 卷：510
• 期：1
• 页码：86-99
• 全文大小：4324 K

文摘

Chitin/chitosan-glucan complex (ChCsGC) was isolated from Schizophyllum commune (S. commune) and dissolved for the first time in precooled (−15 °C) 8 wt.% urea/6 wt.% NaOH aqueous solution. Novel nonwoven microfiber mats were fabricated by wet-dry-spinning technique and evaluated the mechanical of fabrics mats and surface morphology. Isolated and nonwoven mat were characterized employing FTIR-ATR, Optical microscope, TGA, DSC, H/C NMR, SEM and XRD techniques. According to the physical/chemical characterization measurements we can assumed that, the net and the novel dressing mats have the same chemical structure with slightly changes in the thermal stability for the dressing mats.The biological activity of the nonwoven ChCsGC fabric was tested against different types of bacteria exhibiting excellent antibacterial activity. Cell viability of the plain complex and nonwovens mats were evaluated utilizing mouse fibroblast cell line varying concentrations and treatment time. ChCsGC did not show any cytotoxicity against mouse fibroblast cells and the cell-fabrics interaction was also investigated using fluorescence microscope. The novel ChCsGC nonwovens exhibited excellent surgical wound healing ability when tested using rat models.