Determination of content and fatty acid composition of unlabeled phosphoinositide species by thin-layer chromatography and gas chromatography
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- 作者：Sabine Kö ; nig ; Mareike Hoffmann ; Alina Mosblech ; Ingo Heilmann
- 关键词：Phosphoinositides ; Fatty acid composition ; Head group isomers ; Thin-layer chromatography ; Gas chromatography ; Yeast ; Arabidopsis ; BY2
- 刊名：Analytical Biochemistry
- 出版年：2008
- 出版时间：15 July 2008
- 年：2008
- 卷：378
- 期：2
- 页码：197-201
- 全文大小：477 K
文摘
Recent advances in research on the physiological roles of phosphoinositides in eukaryotic organisms indicate a need to distinguish molecular phosphoinositide species on the basis of their characteristic head groups as well as their glycerolipid moieties. Accurate identification of phosphoinositide species in biological samples poses an analytical challenge, because structurally similar inositol phosphate head groups must be resolved, as must lipid-associated fatty acids. Although intact phosphoinositide species have been successfully analyzed, such analyses employ state-of-the-art liquid chromatography/mass spectrometry and require expensive equipment not accessible to many researchers. Described here is a cost-efficient and reliable alternative developed by adaptation of a combination of classic methods for lipid analysis, thin-layer chromatography and gas chromatography.