Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid
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- 作者：Albert Lowe1 ; Raven Harris1 ; Punita Bhansali1 ; Ales Cvekl1 ; 2 ; Wei Liu1 ; 2 ; wei.liu@einstein.yu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：human embryonic stem cells ; differentiation ; extracellular matrix ; epithelialization ; apicobasal cell polarity ; retinal progenitor cells ; self-organizing organoids ; cell adhesion ; Rho kinase (ROCK) ; actomyosin ; cell survival ; cell-surface tension
- 刊名：Stem Cell Reports
- 出版年：2016
- 出版时间：10 May 2016
- 年：2016
- 卷：6
- 期：5
- 页码：743-756
- 全文大小：5602 K
文摘
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Established a method for isolating large amounts of retinal organoids from hESCs
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Dispase-mediated cell detachment led to self-formation of the retinal organoids
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Intercellular adhesions in the floating cultures are required for cell survival
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ROCK-regulated actomyosin-driven forces are required for the self-organization