A novel in vivo inducible dendritic cell ablation model in mice

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• 作者：Megumi Okuyama ; Hisako Kayama ; Koji Atarashi ; Hiroyuki Saiga ; Taishi Kimura ; Ari Waisman ; Masahiro Yamamoto ; Kiyoshi Takeda
• 关键词：Dendritic cells ; Diphtheria toxin receptor ; Cre recombinase ; Immunology
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：2 July 2010
• 年：2010
• 卷：397
• 期：3
• 页码：559-563
• 全文大小：678 K

文摘

Dendritic cells (DCs) are involved in T cell activation via their uptake and presentation of antigens. In vivo function of DCs was analyzed using transgenic mouse models that express diphtheria toxin receptor (DTR) or the diphtheria toxin-A subunit (DTA) under the control of the CD11c/Itgax promoter. However, CD11c⁺ cells are heterogeneous populations that contain several DC subsets. Thus, the in vivo function of each subset of DCs remains to be elucidated. Here, we describe a new inducible DC ablation model, in which DTR expression is induced under the CD11c/Itgax promoter after Cre-mediated excision of a stop cassette (CD11c-iDTR). Crossing of CD11c-iDTR mice with CAG-Cre transgenic mice, expressing Cre recombinase under control of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid promoter, led to the generation of mice, in which DTR was selectively expressed in CD11c⁺ cells (iDTRΔ mice). We successfully deleted CD11c⁺ cells in bone marrow-derived DCs in vitro and splenic CD11c⁺ cells in vivo after DT treatment in iDTRΔ mice. This mouse strain will be a useful tool for generating mice lacking a specific subset of DCs using a transgenic mouse strain, in which the Cre gene is expressed by a DC subset-specific promoter.