Tregs are a promising diagnostic biomarker in transplantation medicine.
Tregs have a potential to support personalized immunosuppressive therapy.
Comparability of results between laboratories is limited due to differences in analytics.
TSDR demethylation qPCR is a non-invasive, reproducible method with sufficient quality for specific detection of nTregs.
Standardization of qPCR for Treg assessment is critical to reduce bias between laboratories.