Changes in the intracellular Ca²⁺ content in human red blood cells in the presence of glycerol

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• 作者：Olga A. Kofanova ; Nina G. Zemlyanskikh ; Lyubomira Ivanova ; Ingolf Bernhardt
• 关键词：Red blood cells ; Intracellular Ca²⁺ ; Fluo-4 ; Glycerol ; Freeze– ; thawing
• 刊名：Bioelectrochemistry
• 出版年：2008
• 出版时间：August 2008
• 年：2008
• 卷：73
• 期：2
• 页码：151-154
• 全文大小：424 K

文摘

Changes of the intracellular Ca²⁺ content in human red blood cells (RBCs) in glycerol-containing solutions and after freeze–thawing the cells with glycerol and subsequent deglycerolization were investigated with the Ca²⁺-sensitive fluorescent dye fluo-4 using fluorescence microscopy. In the glycerol-containing solutions the Ca²⁺ content increased when compared with a physiological medium (Hepes buffered saline solution (HBSS)). This effect was most likely a result of an inhibition of the Ca²⁺ pump. After inhibiting the Ca²⁺ pump using o-vanadate, the Ca²⁺ uptake was not significantly different in the cells in glycerol-containing and physiological medium. Freeze–thawing and deglycerolization of RBCs resulted in a more pronounced increase in the Ca²⁺ content. Also in this case, the Ca²⁺ pump seemed to play a major role.