Dendritic cell-derived TNF-α is responsible for development of IL-10-producing CD4⁺ T cells

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• 作者：Noriyuki Hirata ; Yoshiki Yanagawa ; Masashi Satoh ; Hisako Ogura ; Takashi Ebihara ; Masayuki Noguchi ; Machiko Matsumoto ; Hiroko Togashi ; Tsukasa Seya ; Kazunori Onoé ; Kazuya Iwabuchi
• 关键词：Dendritic cells ; CD4⁺ T cells ; TNF-α ; IL-10 ; Antigen presentation
• 刊名：Cellular Immunology
• 出版年：2010
• 出版时间：2010
• 年：2010
• 卷：261
• 期：1
• 页码：37-41
• 全文大小：434 K

文摘

Immature dendritic cells (DCs) appear to be involved in peripheral immune tolerance via induction of IL-10-producing CD4⁺ T cells. We examined the role of TNF-α in generation of the IL-10-producing CD4⁺ T cells by immature DCs. Immature bone marrow-derived DCs from wild type (WT) or TNF-α^−/− mice were cocultured with CD4⁺ T cells from OVA specific TCR transgenic mice (OT-II) in the presence of OVA_323–339 peptide. The WT DCs efficiently induced the antigen-specific IL-10-producing CD4⁺ T cells, while the ability of the TNF-α^−/− DCs to induce these CD4⁺ T cells was considerably depressed. Addition of exogenous TNF-α recovered the impaired ability of the TNF-α^−/− DCs to induce IL-10-producing T cells. However, no difference in this ability was observed between TNF-α^−/− and WT DCs after their maturation by LPS. Thus, TNF-α appears to be critical for the generation of IL-10-producing CD4⁺ T cells during the antigen presentation by immature DCs.