Characterization of the activity of β-galactosidase from Escherichia coli and Drosophila melanogaster in fixed and non-fixed Drosophila tissues

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• 作者：Mizuki Tomizawa¹ ; Kohei Tsumaki¹ ; Masaki Sone ; ^{masaki.sone@sci.toho-u.ac.jp}
• 关键词：β-Galactosidase ; lacZ ; Drosophila ; X-Gal
• 刊名：Biochimie Open
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：3
• 期：Complete
• 页码：1-7
• 全文大小：695 K
• 卷排序：3

文摘

We created a transgenic fly to express Drosophila endogenous β-galactosidase. We compared the properties of β-galactosidase molecules from Escherichia coli and Drosophila. Both β-galactosidase molecules were active in both fixed and non-fixed tissues. E. coli β-galactosidase showed a preference for neutral pH but not for acidic pH.