GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives
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- 作者：Tim Kao1 ; 2 ; Tanya Labonne1 ; Jonathan C. Niclis4 ; Ritu Chaurasia2 ; Zerina Lokmic1 ; Elizabeth Qian1 ; 2 ; Freya F. Bruveris1 ; 3 ; Sara E. Howden1 ; 2 ; Ali Motazedian1 ; 2 ; Jacqueline V. Schiesser3 ; 8 ; Magdaline Costa9 ; Koula Sourris1 ; Elizabeth Ng1 ; David Anderson1 ; Antonietta Giudice3 ; Peter Farlie1 ; 2 ; Michael Cheung1 ; 2 ; 7 ; Shireen R. Lamande1 ; 2 ; Anthony J. Penington1 ; 2 ; Clare L. Parish4 ; Lachlan H. Thomson4 ; Arash Rafii5 ; 6 ; David A. Elliott1 ; 10 ; Andrew G. Elefanty1 ; 2 ; 3 ; andrew.elefanty@mcri.edu.au" class="auth_mail" title="E-mail the corresponding author ; Edouard G. Stanley1 ; 2 ; 3 ; ed.stanley@mcri.edu.au" class="auth_mail" title="E-mail the corresponding author
- 刊名：Stem Cell Reports
- 出版年：2016
- 出版时间：13 September 2016
- 年：2016
- 卷：7
- 期：3
- 页码：518-526
- 全文大小：2258 K
文摘
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GAPTrap vector system targets transgenes to the ubiquitously expressed GAPDH locus
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Targeting transgenes to the GAPDH locus yields reliable transgene expression
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Transgenes at this locus are robustly expressed in differentiated cells
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Generation of GAPTrap targeted human PSC lines is simple and efficient