LC-MS/MS analysis of chitooligosaccharides

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• 作者：Jaehyun Kim ; Jinhee Kim ; Jangmi Hong ; Sunyoung Lee ; Sehwan Park ; Ji-Hye Lee ; Jeongkwon Kim ; ^{jkkim48105@cnu.ac.kr}
• 关键词：Chitooligosaccharides ; HPLC ; ESI ; Mass spectrometry ; Chitosan
• 刊名：Carbohydrate Research
• 出版年：2013
• 出版时间：3 May, 2013
• 年：2013
• 卷：372
• 期：Complete
• 页码：23-29
• 全文大小：756 K

文摘

Chitooligosaccharides with degrees of polymerization (DPs) 2-12 were successfully separated and characterized using LC-MS/MS analysis with an amine column and a quadrupole ion trap analyzer. Major MS peaks for each chromatographic peak originated from deacetylated chitooligosaccharides while N-acetylated chitooligosaccharides were observed as minor peaks. The ratio of acetylation per glucosamine unit for each chitooligomer was higher for oligomers with higher DPs. The MS/MS spectra of chitooligosaccharides were used to characterize the chitooligosaccharide fragmentation patterns. To the best of our knowledge, this is the first report of on-line LC-MS/MS analysis of chitooligosaccharides with DP higher than 6.