Identification of retroviral conserved pol sequences in serum of mixed connective tissue disease and systemic sclerosis patients

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• 作者：Prokop ; Janusz ; Jagodzinski ; Pawel P.
• 关键词：Antinuclear antibodies ; Endogenous retroviruses ; Mixed connective tissue disease ; Systemic sclerosis
• 刊名：Biomedicine and Pharmacotherapy
• 出版年：2004
• 出版时间：January, 2004
• 年：2004
• 卷：58
• 期：1
• 页码：61-64
• 全文大小：168 K

文摘

Human endogenous retroviruses (HERVs) expression has been suspected as a factor participating in the development of autoimmune diseases. Employing the dot blot hybridisation, we observed that 90.9 % of mixed connective tissue disease (MCTD) (n = 22) patients exhibited the presence of human immunodeficiency virus type 1 (HIV-1) conserved pol sequence in DNA isolated from serum. The same investigation conducted in the group of systemic sclerosis (SSc) (n = 79) patients identified a conserved retroviral pol sequence in 34.6 % of antinuclear antibodies (ANA) positive (n = 52) and 40.7 % of ANA negative (n = 27) patients. However, 100 % of anti-U1-ribonucleoprotein (RNP) positive SSc (n = 9) patients exhibited the presence of HIV-1 conserved pol sequence. Our findings suggest that the presence of retroviral sequence in serum of MCTD and SSc patients may correlate with development of autoimmune response directed against U1-70 kDa polypeptide antigen.