- 作者：Hanna Piotrowska ; Pawel P. Jagodzinski
- 关键词：Epigenetic factors ; Splicing factors ; Glucocorticoids ; Glucocorticoid receptor
- 刊名：Archives of Medical Research
- 出版年：2009
- 出版时间：April 2009
- 年：2009
- 卷：40
- 期：3
- 页码：156-162
- 全文大小：395 K
Background and Aims
Transcriptional activity of NF-κB is inhibited by the liganded glucocorticoid receptor (GR), which exists mainly in two splice variants as functional GRα and nonfunctional GRβ. We investigated the effect of 5-aza-2′-deoxycytidine (5-dAzaC), trichostatin A (TSA), and sodium butyrate (NaBu) on GRα,GRβ and ASF/SF2 splicing factor expression in HT-29 colon and MCF-7 breast carcinoma cells.Methods
HT-29 and MCF-7 cells were cultured in the absence or in the presence of 5-dAzaC, TSA, and NaBu, followed by RNA and protein isolation. The transcript and protein levels of GRα, GRβ ASF/SF2 were determined by reverse transcription, real-time quantitative PCR and Western blot analysis.
Results
We found that 5-dAzaC, TSA, and NaBu lead to an increase in GRα and ASF/SF2 transcript levels and a decrease in GRβ transcript levels in HT-29 and MCF-7 cells. The 5-dAzaC, TSA, and NaBu resulted in increased GRα and ASF/SF2 protein levels and GRβ protein downregulation in HT-29 cells. The most increased GRα protein expression in MCF-7 cells was observed with NaBu. However, all of these compounds inhibited GRβ protein expression in MCF-7 cells. The MCF-7 cells treated with NaBu demonstrated a remarkable increase in ASF/SF2 protein expression.
Conclusions
Because NF-κB is considered to be a factor in the augmentation of malignant properties of cells, treatment of tumors with 5-dAzaC, TSA, and NaBu may provide a novel approach to the enhancement of therapeutic effects of glucocorticoids in epithelial carcinomas.