Hepatic differentiation of human pluripotent stem cells in miniaturized format suitable for high-throughput screen
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- 作者：Arnaud Carpentier ; 1 ; arnaud.carpentier@twincore.de" class="auth_mail" title="E-mail the corresponding author ; Ila Nimgaonkar ; Virginia Chu ; Yuchen Xia ; Zongyi Hu ; T. Jake Liang ; jakel@bdg10.niddk.nih.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pluripotent stem cells ; Hepatic differentiation ; High-throughput assay ; 384-Well plates
- 刊名：Stem Cell Research
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：16
- 期：3
- 页码：640-650
- 全文大小：5268 K
文摘
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Hepatic differentiation of human PSCs in 96- and 384-w plates
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Phenotypic and functional CV% < 15%: suitable for high-throughput assays
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New HLC culture medium: maintenance and further hepatic maturation for > 30 days
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Upon HCV inoculation, detectable intra and extracellular HCV RNA for > 30 days