In vitro

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• 作者：Francielli Chrestani ; Maria Rita Sierakowski ; Daniel Esdras de Andrade Uchoa ; Carlos Nozawa ; Guilherme Lanzi Sassaki ; Philip Albert James Gorin ; Lucy Ono
• 关键词：Galactomannan ; Sulfation ; Mimosa scabrella ; NMR ; Antiviral ; HSV ; Rotavirus
• 刊名：International Journal of Biological Macromolecules
• 出版年：2009
• 出版时间：1 December 2009
• 年：2009
• 卷：45
• 期：5
• 页码：453-457
• 全文大小：488 K

文摘

A chemically sulfated galactomannan (BRS) from seeds of Mimosa scabrella had in vitro antiviral activity against Herpes simplex virus 1 (HSV-1), but not against Simian rotavirus A/SA11 (SiRV-A/SA11). It was examined by ¹³C NMR spectroscopy, which showed the sulfate groups to be mainly at C-6 of galactose residues. BRS had a selective inhibition against HSV-1 during its attachment step, having an IC₅₀ lower than 2.5 μg/ml, determined by plaque reduction, and a selectivity index of greater than 181, suggesting that the antiviral effect is likely due to interactions between the virus and BRS, being influenced its overall surface charge.