Fucomannogalactan and glucan from mushroom Amanita muscaria: Structure and inflammatory pain inhibition
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- 作者：Andrea Caroline Ruthes ; Elaine R. Carbonero ; Marina Machado C¨®rdova ; Cristiane Hatsuko Baggio ; Guilherme Lanzi Sassaki ; Philip Albert James Gorin ; Adair Roberto Soares Santos ; Marcello Iacomini
- 关键词：Amanita muscaria ; Fucomannogalactan ; (1 ; &rarr ; ; 3) ; (1 ; &rarr ; ; 6) ¦Â-d-Glucan ; Anti-inflammatory ; Pain
- 刊名：Carbohydrate Polymers
- 出版年：2013
- 出版时间：15 October, 2013
- 年：2013
- 卷：98
- 期：1
- 页码：761-769
- 全文大小：1028 K
文摘
A fucomannogalactan (FMG-Am) and a (1 ¡ú 3), (1 ¡ú 6)-linked ¦Â-d-glucan (¦ÂGLC-Am) were isolated from Amanita muscaria fruiting bodies. These compounds¡¯ structures were determined using mono- and bi-dimensional NMR spectroscopy, methylation analysis, and controlled Smith degradation. FMG-Am was shown to be a heterogalactan formed by a (1 ¡ú 6)-linked ¦Á-d-galactopyranosyl main chain partially substituted at O-2 mainly by ¦Á-l-fucopyranose and a minor proportion of ¦Â-d-mannopyranose non-reducing end units. ¦ÂGLC-Am was identified as a (1 ¡ú 3)-linked ¦Â-d-glucan partially substituted at O-6 by mono- and a few oligosaccharide side chains, which was confirmed after controlled Smith degradation. Both the homo- and heteropolysaccharide were evaluated for their anti-inflammatory and antinociceptive potential, and they produced potent inhibition of inflammatory pain, specifically, 91 ¡À 8 % (30 mg kg?1) and 88 ¡À 7 % (10 mg kg?1), respectively.