A rapid and non leaky way for preparation of the sharp intracellular recording microelectrodes

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• 作者：Masoud Fereidoni ; Yaghoub Fathollahi ; Mahyar Janahmadi ; Iran Godarzi
• 关键词：Bubbles elimination ; Intracellular recording ; Glass sharp microelectrode
• 刊名：Journal of Biochemical and Biophysical Methods
• 出版年：2008
• 出版时间：24 April 2008
• 年：2008
• 卷：70
• 期：6
• 页码：1124-1129
• 全文大小：464 K

文摘

To fill microelectrodes using backfilling method needs excessive time approximately 4–6  h. It is often difficult to fill microelectrodes without damage or leakage. A main problem is bubble formation in microelectrodes which has an impact on the electrical properties of the electrode and thus it influences the quality of the recording. Based on Archimede's principle there is a force within a solution which pushes insoluble material with a lower specific gravity upward and outside of the solution. Centrifugation can increase the force to eliminate the bubbles.