Development and validation of an ELISA kit (YF MAC-HD) to detect IgM to yellow fever virus
详细信息 查看全文
- 作者：Alison Jane Basile ; ajj1@cdc.gov" class="auth_mail" title="E-mail the corresponding author ; Christin Goodman ; Kalanthe Horiuchi ; Janeen Laven ; Amanda J. Panella ; Olga Kosoy ; Robert S. Lanciotti ; Barbara W. Johnson
- 关键词：Yellow fever virus ; IgM ; ELISA ; Kit ; Diagnostic
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：225
- 期：Complete
- 页码：41-48
- 全文大小：541 K
文摘
- •
A yellow fever IgM ELISA assay kit (YF MAC-HD) based upon the CDC MAC-ELISA was developed.
- •
Assay time is reduced from 2 days to 3.5 h.
- •
The kit is >90% accurate when compared to the CDC MAC-ELISA.
- •
Kits are stored at 4 °C and can tolerate temporary suboptimal storage or shipping conditions.
- •
The kit will reduce materials wastage and improve quality of YFV serology in endemic areas.