Data on the characterization of follicle-stimulating hormone monoclonal antibodies and localization in Japanese eel pituitary

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• 作者：Dae-Jung Kim^a ; Chae-Won Park^b ; Munkhzaya Byambaragchaa^b ; Shin-Kwon Kim^a ; Bae-Ik Lee^a ; Hyung-Kyu Hwang^a ; Jeong-In Myeong^a ; Sun-Mee Hong^c ; Myung-Hwa Kang^d ; Kwan-Sik Min^b ; ^{ksmin@hknu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Japanese eel ; FSH ; Monoclonal Antibody
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：404-410
• 全文大小：4112 K

文摘

Monoclonal antibodies were generated against recombinant follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica; rec-FSH was produced in Escherichia coli and purified using Ni-NTA Sepharose column chromatography.

In support of our recent publication, "Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica" [1], it was important to characterize the specificity of eel follicle-stimulating hormone antibodies. Here, the production and ELISA system of these monoclonal antibodies are presented. The affinity-purified monoclonal antibodies specifically detected eel rec-FSH in ELISA and on western blots of rec-FSH produced from CHO cells. Immunohistochemical analysis revealed that FSH staining was specifically localized in the eel pituitary.