Chain conformation and anti-tumor activity of derivatives of polysaccharide from Rhizoma Panacis Japonici

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• 作者：Cong Chen^a ; Wenhua Wu^a ; Xiaojuan Xu^a ; ^{xuxj@whu.edu.cn" class="auth_mail} ; Lina Zhang^a ; ^{zhangln@whu.edu.cn" class="auth_mail} ; Ying Liu^b ; Kaiping Wang^b
• 关键词：Carboxymethylated polysaccharide ; Sulfated polysaccharide ; Phosphated polysaccharide ; Chain conformation ; Anti-tumor activity
• 刊名：Carbohydrate Polymers
• 出版年：25 May, 2014
• 年：2014
• 卷：105
• 期：Complete
• 页码：308-316
• 全文大小：1977 K

文摘

A hyper branched (1鈫?)-伪-d-glucan (RPS3) with degree of branching of 35% extracted from Rhizoma Panacis Japonici adopted a sphere-like conformation and showed little bioactivity. Three derivatives including sulfated (S-), phosphated (P-) and carboxymethylated (CM-) RPS3 were then synthesized and characterized by FTIR, ¹³CNMR, and SEC-LLS-Vis-RI. As a result, the molecular weights of CM-RPS3, S-RPS3 and P-RPS3 decreased sharply in contrast to the original one, suggesting that chemical degradation has occurred. Moreover, the sphere-like conformation of RPS3 transferred into the random coil-like conformation according to the increased values of 伪_畏, 伪_s and 蟻. It was ascribed to the occurrence of the preferential degradation for the branches. In vitro and in vivo tests demonstrated that the negatively charged S-RPS3 and P-RPS3 with properly low molecular mass significantly inhibited H-22 tumor cells growth. This work offered valuable results for broadening the biological applications of 伪-d-glucans.