- 作者：Balamurugan Chandramohan<sup>1sup> ; Kadarkarai Murugan<sup>1sup> ; Pari Madhiyazhagan<sup>1sup> ; Kalimuthu Kovendan<sup>1sup> ; Palanisamy Mahesh Kumar<sup>1sup> ; Chellasamy Panneerselvam<sup>2sup> ; Devakumar Dinesh<sup>1sup> ; Jayapal Subramaniam<sup>1sup> ; Rajapandian Rajaganesh<sup>1sup> ; Marcello Nicoletti<sup>3sup> ; Angelo Canale<sup>4sup> ; Giovanni Benelli<sup>4sup><sup> ; sup><sup>g.benelli@sssup.it" class="auth_mail" title="E-mail the corresponding authorsup><sup> ; sup><sup>benelli.giovanni@gmail.com" class="auth_mail" title="E-mail the corresponding authorsup>
- 关键词：Arbovirus ; Azadirachta indica ; Biosafety ; Botanical by-products ; Eco-friendly pesticides ; Mosquito-borne diseases
- 刊名：Asian Pacific Journal of Tropical Biomedicine
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：6
- 期：6
- 页码：472-476
- 全文大小：318 K
sSec_2">Methods
spara0015">Neem cake fractions' total methanol extract (NTMeOH), total ethyl acetate extract (NTAcOEt), ethyl acetate fraction after repartition with NTMeOH (NRAcOEt), butanol fraction after repartition with NTMeOH (NRBuOH), and aqueous fraction after repartition of NTMeOH (NRH<sub>2sub>O) were tested against An. culicifacies eggs, fourth instar larvae and adults.
sSec_3">Results
spara0020">In larvicidal experiments, NTMeOH, NTAcOEt, NRAcOEt, NRBuOH and NRH<sub>2sub>O achieved LC<sub>50sub> values of 1.32, 1.50, 1.81, 1.95 and 2.54 mg/L, respectively. All fractions tested at 150 mg/L were able to reduce egg hatchability of more than 50%, with the exception of NTAcOEt and NRAcOEt. In adulticidal assays, NTMeOH, NTAcOEt, NRAcOEt, NRBuOH and NRH<sub>2sub>O achieved LC<sub>50sub> values of 3.01, 2.95, 3.23, 3.63 and 3.00 mg/L, respectively.
sSec_4">Conclusions
spara0025">Overall, this study suggests that the methanolic fractions of neem cake may be considered as a new and cheap source of highly effective compounds against the rural malaria vector An. culicifacies.