Biocompatibility of ferritin-based nanoparticles as targeted MRI contrast agents

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• 作者：Jennifer R. Charlton ; MD ; MSc^a ; ^{jrc6n@virginia.edu" class="auth_mail" title="E-mail the corresponding author} ; Valeria M. Pearl ; BS^a ; ^{vmb3f@virginia.edu" class="auth_mail" title="E-mail the corresponding author} ; Anna R. Denotti ; MD^b ; ^{an.den@hotmail.it" class="auth_mail" title="E-mail the corresponding author} ; Jonathan B. Lee ; MD^c ; ^{jbl5c@virginia.edu" class="auth_mail" title="E-mail the corresponding author} ; Sundararaman Swaminathan ; MD^d ; ^{ss4sa@virginia.edu" class="auth_mail" title="E-mail the corresponding author} ; Yogesh M. Scindia ; PhD^d ; ^{ys9t@virginia.edu" class="auth_mail" title="E-mail the corresponding author} ; Nathan P. Charlton ; MD^e ; ^{npc8a@virginia.edu" class="auth_mail" title="E-mail the corresponding author} ; Edwin J. Baldelomar ; BS^f ; ^{ebaldelo@hawaii.edu" class="auth_mail" title="E-mail the corresponding author} ; Scott C. Beeman ; PhD^h ; ^{beemans@mir.wustl.edu" class="auth_mail" title="E-mail the corresponding author} ; Kevin M. Bennett ; PhD^g ; ^{kevinben@hawaii.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ferritin ; Magnetic resonance imaging ; Toxicity ; Nephron number ; Kidney ; Cationic ferritin
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：12
• 期：6
• 页码：1735-1745
• 全文大小：2211 K

文摘

Ferritin is a naturally occurring iron storage protein, proposed as a clinically relevant nanoparticle with applications as a diagnostic and therapeutic agent. Cationic ferritin is a targeted, injectable contrast agent to measure kidney microstructure with MRI. Here, the toxicity of horse spleen ferritin is assessed as a step to clinical translation. Adult male mice received cationic, native and high dose cationic ferritin (CF, NF, or HDCF) or saline and were monitored for 3 weeks. Transient weight loss occurred in the ferritin groups with no difference in renal function parameters. Ferritin-injected mice demonstrated a lower serum iron 3 weeks after administration. In ferritin-injected animals pre-treated with hydrocortisone, there were no structural or weight differences in the kidneys, liver, lung, heart, or spleen. This study demonstrates a lack of significant detrimental effects of horse-derived ferritin-based nanoparticles at MRI-detectable doses, allowing further exploration of these agents in basic research and clinical diagnostics.