Differential expression of phospholipases D1 and D2 in mouse tissues

详细信息    查看全文

• 作者：Heechul Kim ; Jeeyoung Lee ; Seungjoon Kim ; Min Kyoung Shin ; Do Sik Min ; Taekyun Shin
• 关键词：Phospholipase D ; Immunoprecipitation ; Tissue distribution ; Mouse
• 刊名：Cell Biology International
• 出版年：2007
• 出版时间：February 2007
• 年：2007
• 卷：31
• 期：2
• 页码：148-155
• 全文大小：2496 K

文摘

The differential expression of phospholipase D (PLD) isozymes, which include PLD1 and PLD2, was examined in various murine tissues, including the cerebrum, cerebellum, heart, lung, liver, spleen, stomach, pancreas, ileum, colon, adrenal gland, kidneys, testes, ovaries, and uterus.

In Western blot analysis, only PLD1 was detected in the heart and ovary, while only PLD2 was detected in the pancreas and ileum. Both PLD1 and PLD2 were strongly expressed in the cerebrum, cerebellum, and lung, and both were also expressed in the liver, spleen, stomach, colon, kidney, testes, and uterus.

Immunohistochemistry showed intense PLD immunostaining in the cerebrum, cerebellum, lungs, intestines, and testis, and weak PLD immunostaining in the liver, kidneys, spleen, and heart.

These findings suggest that PLD1 and PLD2 are differentially expressed in the various organs of mice, and that each PLD isozyme plays a distinct role in each organ.