HIV-1 prophylactic vaccine comprised of topical DermaVir prime and protein boost elicits cellular immune responses and controls pathogenic R5 SHIV162P3

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• 作者：Anthony D. Cristillo ; Julianna Lisziewicz ; Leilei He ; Franco Lori ; Lindsey Galmin ; Jeffrey N. Trocio ; Tami Unangst ; Lucia Whitman ; Lauren Hudacik ; Nyasha Bakare ; Stephen Whitney ; Susana Restrepo ; John
• 关键词：HIV-1 ; Vaccine ; AIDS ; Cellular immune response ; T cell memory
• 刊名：Virology
• 出版年：2007
• 出版时间：15 September 2007
• 年：2007
• 卷：366
• 期：1
• 页码：197-211
• 全文大小：1388 K

文摘

Topical DNA vaccination (DermaVir) facilitates antigen presentation to naive T cells. DermaVir immunization in mice, using HIV-1 Env and Gag, elicited cellular immune responses. Boosting with HIV-1 gp120 Env and p41 Gag augmented Th1 cytokine levels. Intramuscular DNA administration was less efficient in priming antigen-specific cytokine production and memory T cells. In rhesus macaques, DermaVir immunization induced Gag- and Env-specific Th1 and Th2 cytokines and generation of memory T cells. Boosting of DermaVir-primed serum antibody levels was noted following gp140(SHIV89.6P)/p27(SIV) immunization. Rectal challenge with pathogenic R5-tropic SHIV162P3 resulted in control of plasma viremia (4/5 animals) that was reflected in jejunum, colon and mesenteric lymph nodes. An inverse correlation was found between Gag- and Env-specific central memory T cell responses on the day of challenge and plasma viremia at set point. Overall, the topical DermaVir/protein vaccination yields central memory T cell responses and facilitates control of pathogenic SHIV infection.