Herpes Virus Infection of RPE and MDCK Cells: Polarity of Infection

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• 作者：TOPP ; KIMBERLY S. ; ROTHMAN ; ALANA L. ; LAVAIL ; JENNIFER H.
• 刊名：Experimental Eye Research
• 出版年：1997
• 出版时间：March, 1997
• 年：1997
• 卷：64
• 期：3
• 页码：343-354
• 全文大小：2.33 M

文摘

Our objective was to determine quantitatively whether herpes simplex virus infects preferentially the apical or basolateral surfaces of two well-differentiated cell types, human retinal pigment epithelial cells and Madin-Darby canine kidney epithelial cells. Secondarily, we sought to localize the mannose 6-phosphate/insulin-like growth factor II receptor, a putative receptor for herpes simplex virus, in the membrane domains of the retinal pigment epithelial cells. Although it has been suggested that receptors utilized by the herpesviruses are heterogeneously distributed on epithelial cells, no quantitative evidence of preferential polarized uptake of wild-type herpes simplex virus into an epithelial cell has yet appeared. Moreover, no evidence has appeared of the distribution of mannose-6-phosphate/insulin-like growth factor II receptor in human retinal pigment epithelial cells. We hypothesized that the preferred pole of uptake and infection by HSV would correlate with the distribution of the receptor. Understanding the preferred site of entry in these cells may shed light on the mechanism of pathological infection and spread of this and related viruses, such as cytomegalovirus, in acute retinal necrosis and herpetic encephalitis.