A zinc fixative for 3D visualization of cerebral capillaries and pericytes
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- 作者：Gitte Nykjæ ; r Nikolajsen
- 关键词：Mice ; Brain ; Capillaries ; Zinc ; Fixative ; Pericytes ; Stereology
- 刊名：Journal of Neuroscience Methods
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：257
- 期：Complete
- 页码：1-6
- 全文大小：1545 K
文摘
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- <p id="par0005">CD31 immunohistochemical staining of zinc-fixated, thick (100 μm) brain tissue resulted in complete visualization of cerebral capillaries in mice.p>
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- <p id="par0010">Fluorescent co-localization of CD31 with the NG2 pericyte marker verified the use of CD31 as a pericyte marker for light microscopic stereological investigations.p>
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- <p id="par0015">The general morphology of zinc-fixated tissue is comparable to aldehyde-based fixatives.p>
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- <p id="par0020">The tissue shrinkage to 30% of the original volume is within an expected normal range.p>