The impact of bioaugmentation on dechlorination kinetics and on microbial dechlorinating communities in subsurface clay till
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- 作者：Jacob B忙lum ; Charlotte Scheutz ; Julie C. Chambon ; Christine Mosegaard Jensen ; Rikke P. Brochmann ; Philip Dennis ; Troels Laier ; Mette M. Broholm ; Poul L. Bjerg ; Philip J. Binning ; Carsten S. Jacobsen
- 关键词：Dechlorination ; Modeling ; qPCR ; Dehalococcoides sp ; Bioaugmentation ; Biostimulation ; KB-1
- 刊名：Environmental Pollution
- 出版年：March, 2014
- 年：2014
- 卷：186
- 期：Complete
- 页码：149-157
- 全文大小：1583 K
文摘
A molecular study on how the abundance of the dechlorinating culture KB-1 affects dechlorination rates in clay till is presented. DNA extracts showed changes in abundance of specific dechlorinators as well as their functional genes. Independently of the KB-1 added, the microbial dechlorinator abundance increased to the same level in all treatments. In the non-bioaugmented microcosms the reductive dehalogenase gene bvcA increased in abundance, but when KB-1 was added the related vcrA gene increased while bvcA genes did not increase. Modeling showed higher vinyl-chloride dechlorination rates and shorter time for complete dechlorination to ethene with higher initial concentration of KB-1 culture, while cis-dichloroethene dechlorination rates were not affected by KB-1 concentrations. This study provides high resolution abundance profiles of Dehalococcoides spp. (DHC) and functional genes, highlights the ecological behavior of KB-1 in clay till, and reinforces the importance of using multiple functional genes as biomarkers for reductive dechlorination.