Alterations in the GyrA and GyrB subunits of topoisomerase II and the ParC and ParE subunits of topoisomerase IV in ciprofloxacin-resistant clinical isolates of Pseudomonas aeruginosa
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- 作者：Lee ; Jeom Kyu ; Lee ; Yeong Seon ; Park ; Yong Keun ; Kim ; Bong Su
- 关键词：P. aeruginosa ; Ciprofloxacin resistance ; GyrA ; GyrB ; ParC ; ParE
- 刊名：International Journal of Antimicrobial Agents
- 出版年：2005
- 出版时间：April, 2005
- 年：2005
- 卷：25
- 期：4
- 页码：290-295
- 全文大小：76.9 K
文摘
The presence of fluoroquinolone resistance-associated alterations in topoisomerase II and IV were investigated for 103 nfxC-like type Pseudomonas aeruginosa isolates. The most nfxC-like type isolates (98.1 % ) possessed the substitution of Ile for Thr-83 in GyrA. A single alteration in GyrA (Thr-83 → Ile) was the most frequently detected and the next common alteration was two alterations with Thr-83 → Ile in GyrA and Ser-87 → Leu in ParC. A novel alteration at position Glin-106 of GyrA, which was suggested to be responsible for fluoroquinolone resistance, was identified. Our study revealed that the alterations in GyrB (Glu-468 → Asp) and in ParE (Asp-419 → Asn or Glu-459 → Asp) play a complementary role in the acquisition of resistance to fluoroquinolone. There was a correlation between the ciprofloxacin MIC and the number of resistance-associated alterations in GyrA, GyrB, ParC and ParE of P. aeruginosa isolates.