Analysis and characterization of hepatitis B vaccine particles synthesized from Hansenula polymorpha

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• 作者：Hyuk-Seong Seo ; Jin-Seung Park ; Kyung-Yeon Han ; Kyung-Dong Bae ; Sang Jeom Ahn ; Hyun Ah Kang ; Jeewon Lee
• 关键词：Hepatitis B surface antigen ; Yeast ; Covalent multimer ; Glycosylation ; Stability
• 刊名：Vaccine
• 出版年：2008
• 出版时间：5 August 2008
• 年：2008
• 卷：26
• 期：33
• 页码：4138-4144
• 全文大小：737 K

文摘

The biochemical and physical properties of hepatitis B virus (HBV) small surface antigen (S-HBVsAg) from Berna Biotech Korea Corp. were systematically analyzed and characterized. Through various electrophoresis and immunoblotting assay of S-HBVsAg and its proteolytic products, it was confirmed that the S-HBVsAg vaccine particles are present in the form of covalent multimers that are assembled via strong intermolecular disulfide bonds. The S-HBVsAg particles contain no N-glycosylation moiety but some O-glycosidically linked mannoses. Evidently from N-terminus sequencing of both monomers and dimers that are formed by complete and partial reduction, respectively, of the S-HBVsAg particles under reducing SDS-PAGE condition, it is evident that each polypeptide within S-HBVsAg particles has authentic sequence of N-terminus. Denaturation plot shows that the S-HBVsAg vaccine particles were extremely stable especially in the solution with high acidity. This stability property of S-HBVsAg vaccine particles could provide very useful information for the optimization of the downstream process of recombinant S-HBVsAg particles synthesized from yeast cultures.