Intestinal anti-inflammatory activity of the seeds of Raphanus sativus L. in experimental ulcerative colitis models

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• 作者：Ki-Choon Choi^a ; ¹ ; ^{choiwh@korea.kr" class="auth_mail" title="E-mail the corresponding author} ; Seong-Wan Cho^b ; ¹ ; ^{swcho@konyang.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Sung-Ho Kook^c ; ^d ; ¹ ; ^{kooksh@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Sa-Ra Chun^c ; ^{tkfkcjs@naver.com" class="auth_mail" title="E-mail the corresponding author} ; Govinda Bhattarai^d ; ^{govinda@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Sher Bahadur Poudel^d ; ^{poudelsb@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Min-Kook Kim^d ; ^{dark-nebula@naver.com" class="auth_mail" title="E-mail the corresponding author} ; Kyung-Yeol Lee^d ; ^{kyleecnu@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Jeong-Chae Lee^c ; ^d ; ^{leejc88@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Raphanus sativus L. ; Experimental colitis ; TNBS ; DSS ; Intestinal anti-inflammation
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：17 February 2016
• 年：2016
• 卷：179
• 期：Complete
• 页码：55-65
• 全文大小：6825 K

文摘

Water extract of Raphanus sativus L. (RSL) seeds was traditionally used to treat digestive inflammatory complaints in Korean culture. RSL seeds exerted antioxidant, anti-inflammatory, and anti-septic functions, suggesting their pharmacological potential for the treatment of inflammatory pathologies associated with oxidative stress such as inflammatory bowel disease.

Aim of this study

We evaluated the intestinal anti-inflammatory effects of RSL seed water extract (RWE) in experimental rat models of trinitrobenzenesulphonic acid (TNBS)- or dextran sodium sulfate (DSS)-induced colitis.

Materials and methods

RWE was characterized by determining the content of sinapic acid as a reference material and then assayed in the DSS and TNBS models of rat colitis. Male Sprague–Dawley rats were divided into 10 groups (n=7/group): non-colitic control, DSS or TNBS control, DSS colitis groups treated with RWE (100 mg/kg) or mesalazine (25 mg/kg), and TNBS colitis groups treated with various doses (10, 40, 70, and 100 mg/kg) of RWE or mesalazine (25 mg/kg). RWE or mesalazine treatment started the same day of colitis induction and rats were sacrificed 24 h after the last treatment followed by histological and biochemical analyses.

Results

Oral administration with RWE suppressed intestinal inflammatory damages in both DSS- and TNBS-induced colitic rats. The treatment with 100 mg/kg RWE recovered intestinal damages caused by TNBS or DSS to levels similar to that of mesalazine, decreasing the activity of myeloperoxidase activity and the secretion of tumor necrosis factor (TNF)-α and interleukin (IL)-1β. RWE treatment inhibited malondialdehyde production and glutathione reduction in colon of colitis rats. The administration of RWE at dose of 100 mg/kg also suppressed the TNBS- or DSS-stimulated expression of TNF-α, IL-1β, monocyte chemotactic protein-1, inducible nitric oxide, and intercellular adhesion molecule-1. Furthermore, RWE inhibited p38 kinase and DNA–nuclear factor-κB binding activities, both of which were stimulated in the colitic rats.

Conclusions

The current findings show that RWE ameliorates intestinal oxidative and inflammatory damages in DSS and TNBS models of rat colitis, suggesting its beneficial use for the treatment of intestinal inflammatory disorders.