Triclosan (TCS) and Triclocarban (TCC) cause lifespan reduction and reproductive impairment through oxidative stress-mediated expression of the defensome in the monogonont rotifer (Brachionus koreanus)

详细信息    查看全文

• 作者：Jeonghoon Han^a ; ¹ ; Eun-Ji Won^a ; ¹ ; Un-Ki Hwang^b ; Il-Chan Kim^c ; Joung Han Yim^c ; Jae-Seong Lee^a ; ^{jslee2@skku.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Triclosan (TCS) ; Triclocarban (TCC) ; Rotifer ; Brachionus koreanus ; Reactive oxygen species
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2016
• 出版时间：July-August 2016
• 年：2016
• 卷：185-186
• 期：Complete
• 页码：131-137
• 全文大小：836 K

文摘

Triclosan (TCS) and Triclocarban (TCC) are used as antimicrobial agents and have been widely dispersed and detected in the marine environment. However, the toxicities of TCS and TCC have been poorly investigated in marine invertebrates. In this study, the effects of TCS and TCC on mortality, population growth, lifespan, and fecundity were examined in the monogonont rotifer (Brachionus koreanus) using cellular ROS levels, GST enzymatic activity, and gene expression of defensomes. The median lethal concentration (LC50) of TCS (393.1 μg/L) and TCC (388.1 μg/L) was also determined in the same species. In TCS- and TCC-exposed B. koreanus, growth retardation and reduced fecundity were observed and were shown to have a potentially deleterious effect on the life cycle of B. koreanus. In addition, time-dependent increases in ROS content (%) and GST enzymatic activity were shown in response to TCS and TCC exposure. Additionally, transcript levels of detoxification proteins (e.g., CYPs), antioxidant proteins (e.g., GST-sigma, Cu/ZnSOD, CAT), and heat shock proteins (Hsps) were modulated in response to TCS and TCC exposure over a 24 h period. Our results indicate that TCS and TCC induce oxidative stress and transcriptional regulation of detoxification, antioxidant, and heat shock proteins, resulting in changes in lifespan and fecundity.