Black ginseng inhibits ethanol-induced teratogenesis in cultured mouse embryos through its effects on antioxidant activity
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- 作者：Se-Ra Lee ; Mi-Ra Kim ; Jung-Min Yon ; In-Jeoung Baek ; Chun Gui Park ; Beom Jun Lee ; Young Won Yun ; Sang-Yoon Nam
- 关键词：Black ginseng ; Ethanol ; Whole embryo culture ; Teratogenesis ; Antioxidant enzyme
- 刊名：Toxicology in Vitro
- 出版年：2009
- 出版时间：February 2009
- 年：2009
- 卷：23
- 期：1
- 页码：47-52
- 全文大小：603 K
文摘
Fetal alcohol syndrome is caused by excessive ethanol consumption during pregnancy. We investigated the effect of black ginseng (red ginseng that is subjected to 9 cycles of 95–100 °C for 2–3 h) on ethanol-induced teratogenesis using an in vitro whole embryo culture system. Postimplantational mouse embryos at embryonic day 8.5 were exposed to ethanol (1 μl/ml) in the presence or absence of black ginseng (1, 10, and 100 μg/ml) for 2 days, and then morphological scoring and real-time PCR analysis were carried out. In ethanol-treated embryos, the total morphological score and individual scores for flexion, heart, fore-, mid-, and hindbrains, otic, optic, and olfactory systems, branchial bars, maxillary and mandibular processes, caudal neural tube, and somites were significantly lower than the control group (p < 0.05). Treatment with black ginseng improved most of the morphological scores significantly as compared to ethanol-treated embryos (p < 0.05). The mRNA levels of the antioxidant enzymes cytosolic glutathione peroxidase (GPx), phospholipid hydroperoxide GPx, and selenoprotein P were significantly decreased in ethanol-treated embryos, but co-treatment with black ginseng restored the mRNA levels to those of control embryos. These results indicate that black ginseng has a protective effect on ethanol-induced teratogenesis through the augmentation of antioxidative activity in embryos.