Validation of biomarkers in cardiotoxicity induced by Periplocin on neonatal rat cardiomyocytes using UPLC-Q-TOF/MS combined with a support vector machine
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- 作者：Aizhu Lia ; Xuejun Guoa ; Jiabin Xiea ; Xinyu Liua ; Zhenzhu Zhanga ; Yubo Lia ; yaowufenxi001@sina.com" class="auth_mail" title="E-mail the corresponding author ; Yanjun Zhangb
- 关键词：Periplocin ; Metabolomics ; UPLC-Q-TOF/MS ; Cardiotoxicity ; Biomarkers ; SVM
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：10 May 2016
- 年：2016
- 卷：123
- 期：Complete
- 页码：179-185
- 全文大小：1762 K
文摘
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Periplocin-induced cardiotoxicity on neonatal rat cardiomyocytes was analyzed by UPLC-Q-TOF/MS-based metabolomics.
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Significant decline was observed in these biomarkers of Periplocin-induced cardiotoxicity.
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A “supervised” Support Vector Machine (SVM) study was used to optimize and verify the collected biomarkers.
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Relevant metabolic pathways of these biomarkers were analyzed by MetPA.
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An UPLC-Q-TOF/MS-based metabolomic approach is a powerful tool for assessing the toxicity of traditional Chinese medicine and drug safety screening.