A reporter classical swine fever virus (CSFV) stably expressing the Renilla luciferase was generated.
The reporter virus showed a growth curve indistinguishable from that of the parent virus.
The reporter virus together with siRNAs was used to screen anti-CSFV ISGs from 21 candidates.
Four potent anti-CSFV ISGs, NRAMP1, NT5C3A, OAS1, and CXCL10, were identified and verified.