Magnetic induction heating of superparamagnetic nanoparticles during rewarming augments the recovery of hUCM-MSCs cryopreserved by vitrification

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• 作者：Jianye Wang^a ; Gang Zhao^a ; ^b ; ^{ZhaoG@ustc.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Zhengliang Zhang^c ; Xiaoliang Xu^d ; Xiaoming He^e ; ^{he.429@osu.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Magnetic induction heating ; Fe3O4 nanoparticles ; Cryopreservation ; Vitrification ; hUCM-MSCs
• 刊名：Acta Biomaterialia
• 出版年：2016
• 出版时间：15 March 2016
• 年：2016
• 卷：33
• 期：Complete
• 页码：264-274
• 全文大小：3286 K

文摘

Cryopreservation by vitrification has been recognized as a promising strategy for long-term banking of living cells. However, the difficulty to generate a fast enough heating rate to minimize devitrification and recrystallization-induced intracellular ice formation during rewarming is one of the major obstacles to successful vitrification. We propose to overcome this hurdle by utilizing magnetic induction heating (MIH) of magnetic nanoparticles to enhance rewarming. In this study, superparamagnetic (SPM) Fe₃O₄ nanoparticles were synthesized by a chemical coprecipitation method. We successfully applied the MIH of Fe₃O₄ nanoparticles for rewarming human umbilical cord matrix mesenchymal stem cells (hUCM-MSCs) cryopreserved by vitrification. Our results show that extracellular Fe₃O₄ nanoparticles with MIH may efficiently suppress devitrification and/or recrystallization during rewarming and significantly improve the survival of vitrified cells. We further optimized the concentration of Fe₃O₄ nanoparticles and the current of an alternating current (AC) magnetic field for generating the MIH to maximize cell viability. Our results indicate that MIH in an AC magnetic field with 0.05% (w/v) Fe₃O₄ nanoparticles significantly facilitates rewarming and improves the cryopreservation outcome of hUCM-MSCs by vitrification. The application of MIH of SPM nanoparticles to achieve rapid and spatially homogeneous heating is a promising strategy for enhanced cryopreservation of stem cells by vitrification.

Statement of Significance

Here we report the successful synthesis and application of Fe₃O₄ nanoparticles for magnetic induction heating (MIH) to enhance rewarming of vitrification-cryopreserved human umbilical cord matrix mesenchymal stem cells (hUCM-MSCs). We found that MIH-enhanced rewarming greatly improves the survival of vitrification-cryopreserved hUCM-MSCs. Moreover, the hUCM-MSCs retain their intact stemness and multilineage potential of differentiation post cryopreservation by vitrification with the MIH-enhanced rewarming. Therefore, the novel MIH-enhanced cell vitrification is valuable to facilitate the long-term storage of hUCM-MSCs and possibly many other important cells to meet their ever-increasing demand by the burgeoning cell-based medicine.