Effects of xenogeneic adipose-derived stem cell transplantation on acute-on-chronic liver failure

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• 作者：Wei Zhu^a ; ^b ; Xiao-Lei Shi^c ; ^d ; Jiang-Qiang Xiao^c ; ^d ; Guang-Xiang Gu^d ; Yi-Tao Ding^c ; ^d ; Zheng-Liang Ma^b ; ^{mazhengliang1964@yahoo.com.cn}
• 关键词：adipose-derived stem cells ; acute-on-chronic liver failure ; cell transplantation
• 刊名：Hepatobiliary & Pancreatic Diseases International
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：12
• 期：1
• 页码：60-67
• 全文大小：4726 K

文摘

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Background

Adipose-derived stem cells (ADSCs) are particularly attractive in future clinical applications of stem cell-based therapy for acute-on-chronic liver failure (ACLF). This study was undertaken to evaluate the therapeutic potential of ADSCs on ACLF.

Methods

ADSCs isolated from porcine fat tissue were expanded and labeled with BrdU. Rabbit models of ACLF were created by administration of D-Gal following CCl₄-induced cirrhosis. One day after administration of D-Gal, rabbits of the ACLF/ADSCs group (n=15) were received ADSCs transplantation, while those in the ACLF/saline group (n=15) were treated with the same volume of saline. Biochemical parameters and histomorphological scoring were evaluated; the distribution and characteristics of transplanted ADSCs as well as the pathology of the liver were examined.

Results

ADSCs transplantation improved the survival rate and the liver function of rabbits with ACLF. Biochemical parameters of the ACLF/ADSCs group were improved compared with those of the ACLF/saline group, and histomorphological scoring of the ACLF/ADSCs group was significantly lower than that of the ACLF/saline group. ADSCs were identified in the periportal region of the liver after cell transplantation.

Conclusion

Xenogenic ADSCs have therapeutic efficacy in the ACLF rabbit model.