Autophagy-related gene16L2, a potential serum biomarker of multiple sclerosis evaluated by bead-based proteomic technology

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• 作者：Linlin Yin^a ; ¹ ; ^{yinll913@126.com" class="auth_mail} ; Jianghong Liu^b ; ¹ ; Huiqing Dong^b ; Erhe Xu^b ; Yuchen Qiao^b ; Lin Wang^b ; Lan Zhang^a ; Jianping Jia^b ; Lin Li^a ; ^{linli97@hotmail.com" class="auth_mail} ; Xingchao Geng^c ; ¹ ; ^{gengxch@nicpbp.org.cn" class="auth_mail}
• 关键词：Atg16L2 ; multiple sclerosis ; proteomic technology ; T cells
• 刊名：Neuroscience Letters
• 出版年：6 March, 2014
• 年：2014
• 卷：562
• 期：Complete
• 页码：34-38
• 全文大小：608 K

文摘

Multiple sclerosis (MS) is an autoimmune disease characterized by neuroinflammation and demyelination that are mediated by T cells. The prolonged survival of autoreactive T cells acts as a primary event to trigger an inflammatory cascade that mediates myelin loss and clinical relapse in MS. Recently, T cell survival has been shown to be modulated by the autophagy-related gene (Atg). In the present study, we performed bead fractionation/matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry analyses using serum from 54 MS patients and 55 healthy controls. Eleven peptides were significantly different between the two groups with one being identified as a fragment of Atg16L2. Then the decreased levels of Atg16L2 peptides in MS patients were validated by immunoblotting and real-time PCR. As the Atg12-Atg5路Atg16 multimeric complex plays an essential role in autophagy, our results suggest that Atg16L2 may play an important role in autophagy of T cells and serve as a potential biomarker to predict clinical relapse of MS.