Identification of a cobia (Rachycentron canadum) CC chemokine gene and its involvement in the inflammatory response

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• 作者：Youlu Su ^{vetsyl@163.com} ; Zhixun Guo ^{guozhixun1@163.com} ; Liwen Xu ^lwxu@163.com ; Jingzhe Jiang ^{jingzhejiang@gmail.com} ; Jiangyong Wang ^{wjy104@163.com} ; Juan Feng ; ^{juanf@21cn.com}
• 关键词：Cobia Rachycentron canadum ; CC chemokine ; Cloning ; Gene expression ; Inflammatory response
• 刊名：Fish & Shellfish Immunology
• 出版年：2012
• 出版时间：January 2012
• 年：2012
• 卷：32
• 期：1
• 页码：204-210
• 全文大小：863 K

文摘

The chemokines regulate immune cell migration under inflammatory and physiological conditions. We investigated a CC chemokine gene (RcCC1) from cobia (Rachycentron canadum). The full-length RcCC1 cDNA is comprised 673 nucleotides and encodes a four-cysteine arrangement 99-amino-acid protein typical of known CC chemokines. The genomic DNA of RcCC1 consists of three exons and two introns. Phylogenetic analysis showed that RcCC1 was closest to the MIP group of CC chemokines. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed RcCC1 was constitutively expressed in all tissues examined, with relative strong expression in gill, blood, kidney, spleen, and head kidney. The RcCC1 transcripts in the head kidney, spleen, and liver were quickly up-regulated after stimulation with formalin-inactivated Vibrio carchariae (bacterial vaccine) or polyriboinosinic polyribocytidylic acid (poly I:C). These results indicate RcCC1 not only plays a role in homeostasis, but also may be involved in inflammatory responses to bacterial and viral infection.