MiR-143 enhances adipogenic differentiation of 3T3-L1 cells through targeting the coding region of mouse pleiotrophin

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• 作者：Can  ; Yi^a ;   ; ^b ;   ; ¹ ; Wei-dong  ; Xie^b ;   ; ¹ ; Fu  ; Li^a ;   ; ^b ;   ; ¹ ; Qing  ; Lv^a ;   ; ^b ; Jie  ; He^a ;   ; ^b ; Jiangbin  ; Wu^a ;   ; ^b ; Dayong  ; Gu^c ; Naihan  ; Xu^b ;   ; ^{xu.naihan@sz.tsinghua.edu.cn} ; Yaou  ; Zhang^b ;   ; ^{zhangyo@sz.tsinghua.edu.cn}
• 关键词：Mir-143 ; Pleiotrophin ; 3T3-L1 preadipocyte ; Adipogenesis ; Rare codon
• 刊名：FEBS Letters
• 出版年：2011
• 出版时间：20 October 2011
• 年：2011
• 卷：585
• 期：20
• 页码：3303-3309
• 全文大小：1085 K

文摘

Adipogenic differentiation of preadipocytes is a complex process regulated by various factors including miRNAs and cytokines. MiR-143 is a well known miRNA that enhances adipogenesis. Pleiotrophin (PTN), a heparin-binding growth factor, plays a negative role in adipogenesis. In this investigation, we demonstrate that PTN is a target gene of miR-143 during adipogenic differentiation in 3T3-L1 preadipocytes. MiR-143 down regulates PTN expression through interaction with a target site of miR-143 in the coding region of mouse PTN. The rare codons upstream of the target site regulate miR143-induced translational knockdown of PTN, which provides more insight into the mechanism of adipogenic differentiation.