Determination of sulfonamides in food samples by membrane-protected micro-solid phase extraction coupled with high performance liquid chromatography

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• 作者：Jiangeng Huang ; Juanjuan Liu ; Cong Zhang ; Jiaojiao Wei ; Li Mei ; Shan Yu ; Gao Li ; ^{ligaotj@163.com} ; Li Xu ; ^{xulpharm@mails.tjmu.edu.cn}
• 关键词：Orthogonal array design ; Membrane-protected micro-solid phase extraction ; Sulfonamides ; Food analysis
• 刊名：Journal of Chromatography A
• 出版年：2012
• 出版时间：6 January, 2012
• 年：2012
• 卷：1219
• 期：Complete
• 页码：66-74
• 全文大小：943 K

文摘

In the present study, a simple and sensitive extraction method based on polypropylene membrane-protected micro-solid phase extraction (MP-¦Ì-SPE) has been developed for analysis of sulfonamides in food samples. Poly (methacrylic acid-ethylene glycol dimethacrylate) (p-MAA-EDMA) was synthesized using orthogonal array experimental design, optimized with three factors at four levels and evaluated on yield, hydrophobic and cation-exchange properties. The optimized p-MAA-EDMA was then employed as the sorbent in the MP-¦Ì-SPE for extraction of sulfonamides from milk and chicken muscle samples, followed by high performance liquid chromatographic analysis with ultraviolet detection. Under optimized extraction conditions, good linearities (0.010-1.0 ¦Ìg mL^? with r² > 0.9900), low limits of detection (0.38-0.62 ng mL^?), and acceptable intra-day (2.7-13.7 % ) and inter-day (6.7-15.2 % ) relative standard deviations were obtained. It was demonstrated to be an effective approach to handle semi-solid/solid samples with good resistance to interference from ¡°dirty?samples.