Post-pellet-digestion precipitation and solid phase extraction: A practical and efficient workflow to extract surrogate peptides for ultra-high performance liquid chromatography - tandem mass spectrometry bioanalysis of a therapeutic antibody in the low ng/mL range
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- 作者：Chao Gong ; Naiyu Zheng ; naiyu.zheng@bms.com" class="auth_mail" title="E-mail the corresponding author ; Jianing Zeng ; jianing.zeng@bms.com" class="auth_mail" title="E-mail the corresponding author ; Anne-Franç ; oise Aubry ; Mark E. Arnold
- 关键词：UHPLC&ndash ; MS/MS ; Pellet digestion ; Solid phase extraction ; Therapeutic antibody ; Protein quantitation ; Matrix effect
- 刊名：Journal of Chromatography A
- 出版年：2015
- 出版时间：11 December 2015
- 年：2015
- 卷：1424
- 期：Complete
- 页码：27-36
- 全文大小：927 K
文摘
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Improved assay sensitivity for LC–MS bioanalysis of proteins using new SPE workflow.
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Employed post-pellet-digestion precipitation to overcome sample clogging during SPE.
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Validated an LC–MS/MS assay for a monoclonal antibody in serum with LLOQ at 50 ng/mL.
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The method was 100-fold more sensitive than a previously reported method without SPE.
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This SPE methodology is applicable to LC–MS/MS bioanalysis of other proteins.