Mating type and ploidy effect on the β-glucosidase activity and ethanol-producing performance of Saccharomyces cerevisiae with multiple δ-integrated bgl1 gene
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- 作者：Jianjun Wanga ; b ; wjj2013@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yuanyuan Maa ; myy@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Kun Zhanga ; zhangkun@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Huajun Yanga ; b ; huajunyang@163.com" class="auth_mail" title="E-mail the corresponding author ; Cheng Liua ; liuc@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Shaolan Zoua ; b ; slzhou@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Jiefang Honga ; hjf@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Minhua Zhanga ; c ; mhzhang@tju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：β-Glucosidase ; δ-Integration ; Ploidy ; Mating-type ; Ethanol
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 August 2016
- 年：2016
- 卷：231
- 期：Complete
- 页码：24-31
- 全文大小：1523 K
文摘
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BGL-a with 19 copies δ-integrated bgl was gained and then seven ploidy series strains.
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Mating type effect: specific activity of BGL-aα was lower than that of BGL-aa or -αα.
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Maximum OD660 decreased with ploidy and specific activity increased with ploidy.
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No typical mating type and ploidy effect on ethanol production in 10% cellobiose.
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BGL-aα produced ethanol (titer 35.65 g/L and yield 83.69%) from pretreated corncob.