Prenatal stress on the kinetic properties of Ca²⁺ and K⁺ channels in offspring hippocampal CA3 pyramidal neurons

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• 作者：Qing Cai ; Zhongliang Zhu ; Hui Li ; Xiaoli Fan ; Ning Jia ; Zhuanli Bai ; Liang Song ; Xia Li ; Jiankang Liu
• 关键词：Prenatal stress ; Patch cl CA3 neurons ; Hippocampus ; Ca²⁺ channels
• 刊名：Life Sciences
• 出版年：2007
• 出版时间：23 January 2007
• 年：2007
• 卷：80
• 期：7
• 页码：681-689
• 全文大小：631 K

文摘

Prenatal stress is known to cause neuronal loss and oxidative damage in the hippocampus of offspring rats. To further understand the mechanisms, the present study was undertaken to investigate the effects of prenatal stress on the kinetic properties of high-voltage-activated (HVA) Ca²⁺ and K⁺ channels in freshly isolated hippocampal CA3 pyramidal neurons of offspring rats. Pregnant rats in the prenatal stress group were exposed to restraint stress on days 14–20 of pregnancy three times daily for 45 min. The patch clamp technique was employed to record HVA Ca²⁺ and K⁺ channel currents. Prenatal stress significantly increased HVA Ca²⁺ channel disturbance including the maximal average HVA calcium peak current amplitude (− 576.52 ± 7.03 pA in control group and − 702.05 ± 6.82 pA in prenatal stress group, p < 0.01), the maximal average HVA Ca²⁺ current density (− 40.89 ± 0.31 pA/pF in control group and − 49.44 ± 0.37 pA/pF in prenatal stress group, p < 0.01), and the maximal average integral current of the HVA Ca²⁺ channel (106.81 ± 4.20 nA ms in control group and 133.49 ± 4.59 nA ms in prenatal stress group, p < 0.01). The current–voltage relationship and conductance—voltage relationship of HVA Ca²⁺ channels and potassium channels in offspring CA3 neurons were not affected by prenatal stress. These data suggest that exposure of animals to stressful experience during pregnancy can exert effects on calcium ion channels of offspring hippocampal neurons and that the calcium channel disturbance may play a role in prenatal stress-induced neuronal loss and oxidative damage in offspring brain.