miR-1182 inhibits growth and mediates the chemosensitivity of bladder cancer by targeting hTERT

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• 作者：Jun Zhou^a ; Wenbin Dai^a ; ^{daiwenbin271@163.com" class="auth_mail" title="E-mail the corresponding author} ; Jianming Song^b
• 关键词：miR-1182 ; hTERT ; Bladder cancer ; Chemosensitivity
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：5 February 2016
• 年：2016
• 卷：470
• 期：2
• 页码：445-452
• 全文大小：2775 K

文摘

microRNAs (miRNAs) have been demonstrated to contribute to tumor progression and metastasis and proposed to be key regulators of diverse biological processes. In this study, we report that miR-1182 is deregulated in bladder cancer tissues and cell lines. To characterize the role of miR-1182 in bladder cancer cells, we performed functional assays. The overexpression of miR-1182 significantly inhibits bladder cancer cell proliferation, colony formation, and invasion. Moreover, its up-regulation induced cell cycle arrest and apoptosis and mediated chemosensitivity to cisplatin in bladder cancer. Furthermore, a luciferase reporter assay and a rescue experiment indicated that miR-1182 directly targets hTERT by binding its 3′UTR. In conclusion, these results demonstrate that miR-1182 acts as a tumor suppressor and may be a potential biomarker for bladder cancer diagnosis and treatment.