Alendronate significantly prevented development of chronic constriction injury–induced mechanical/thermal hypersensitivity, after intrathecal and intra-peritoneal administration.

Chronic constriction injury–induced microglial activation and phosphorylation of p38 in the spinal cord were attenuated by alendronate administration.

Alendronate downregulated phosphorylated p38 expression and decreased the expression of inflammatory cytokines (tumor necrosis factor α, and interleukins 1β and 6) in lipopolysaccharide-stimulated primary microglia culture.