Metabolite variations between acute-on-chronic liver failure and chronic liver failure caused by hepatitis B virus based on ultra-performance liquid chromatography mass spectrometry

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• 作者：Jiangshan Lian¹ ; Xiaofen Li¹ ; Yinyin Wang ; Jianle Yang ; Wei Liu ; Jing Ma ; Deying Chen ; Lanjuan Li ; Jianrong Huang ; ^{hzhuangchina@sina.com}
• 关键词：Acute-on-chronic liver failure ; Chronic liver failure ; Metabolomics ; Multivariate analysis ; Ultra-performance liquid chromatography
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：994-1000
• 全文大小：1234 K
• 卷排序：84

文摘

The present study aims to compare serum metabolite alterations between acute-on-chronic liver failure (ACLF) and chronic liver failure (CLF), and find the specific biomarkers associated with the diseases.MethodsSerum samples were collected from patients with ACLF (n = 76) and CLF (n = 56) as well as healthy individuals (n = 20) and assayed by ultra-performance liquid chromatography mass spectrometry (UPLC–MS). The acquired data was analyzed using principal components analysis (PCA) and partial least squares discriminate analysis (PLS-DA).ResultsThe PLS-DA model with satisfactory explanatory and predictive ability (R2 = 0.979, Q2 = 0.918) is capable of discriminate ACLF patients from CLF patients. Significant difference in the metabolomics among the three groups was observed, metabolites that decreased significantly in the serum of ACLF and CLF included phosphatidylcholines (PCs) and lysophosphatidylcholines (LPCs), whereas conjugated bile acids (GCDCA, GUDCA) increased significantly, these metabolites considered as common biomarkers of liver failure. Linoleyl carnitine showed significant increase in CLF compared with controls while no significant change was observed in ACLF, it could be special biomarkers of ACLF and CLF.ConclusionMetabolomics based on ultra-performance liquid chromatography mass spectrometry provide a new way to diagnose and reveal the pathogenesis of ACLF and CLF.