The evaluation of 7 commercial real-time PCR kits for Zaire ebolavirus using virus-like particle-encapsulated EBOV RNA
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- 作者：Guojing Wanga ; b ; Rui Zhanga ; Yanxi Hana ; Le Changa ; b ; Dong Zhanga ; b ; Lei Zhanga ; c ; Xin Yanga ; b ; Chao Liua ; b ; Jiansheng Dinga ; b ; Kuo Zhanga ; Guigao Lina ; Xie Jiehonga ; Jinming Lia ; b ; jmli@nccl.org.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ebola virus ; Kits evaluation ; Real time RT-PCR ; Virus-like particles
- 刊名：Diagnostic Microbiology & Infectious Disease
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：83
- 期：4
- 页码：355-358
- 全文大小：215 K
文摘
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Several real-time PCR kits for Zaire ebolavirus have been evaluated in China.
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Virus-like particles that encapsulated EBOV RNA have been prepared as positive substitutes.
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The limit of detection and the precision were completed for the assays.
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Requirements for clinical EBOV detection are basically satisfied except 1 reagent.
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It is feasible for virus-like particles as a mimic of positive samples in assay evaluation.