Diagnosis of renal allograft subclinical rejection by urine protein fingerprint analysis

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• 作者：Youying Mao ; Jiekai Yu ; Jianghua Chen ; Hao Yang ; Qiang He ; Zhangfei Shou ; Jianyong Wu ; Shu Zheng
• 关键词：Protein fingerprint ; Subclinical rejection ; Diagnosis
• 刊名：Transplant Immunology
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：18
• 期：3
• 页码：255-259
• 全文大小：395 K

文摘

This study aimed to find new biomarkers and establish urine protein fingerprint model for diagnosis of renal allograft subclinical rejection (SCR).

Methods

A total of 73 urine samples were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) combined with bioinformatics tools.

Results

Firstly, 22 urine samples from recipients of stable graft function proved by protocol biopsies and 27 from subclinical rejection gruop were analyzed by SELDI-TOF-MS and Zhejiang University Cancer Institute — ProteinChip Data Analysis System (ZUCI-PDAS). The diagnostic pattern comprised of 4 biomarkers could differentiate SCR group from stable group with sensitivity of 81.5 % and specificity of 81.8 % . The remaining 14 samples from stable group and 10 samples from SCR were analyzed on the second day as an independent test set. The independent tests yielded a specificity of 71.4 % and sensitivity of 90 % .

Conclusions

Urine protein fingerprint analysis by SELDI-TOF-MS combined with bioinformatics can help to discover new biomarkers and provide a non-invasive tool to diagnosis of SCR.