Genetic features of livestock-associated Staphylococcus aureus ST9 isolates from Chinese pigs that carry the lsa(E) gene for quinupristin/dalfopristin resistance

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• 作者：Xiaomei Yan^a ; ^d ; ¹ ; Zongwei Li^b ; ¹ ; Monika A. Chlebowicz^c ; Xiaoxia Tao^a ; ^d ; Ming Ni^b ; Yuan Hu^a ; Zhen Li^b ; Hajo Grundmann^h ; Susan Murray^f ; Ben Pascoe^f ; ^g ; Samuel K. Sheppard^f ; ^g ; Xiaochen Bo^b ; Jan Maarten van Dijl^c ; Pengcheng Du^a ; ^d ; Minli Zhang^b ; Yuanhai You^a ; ^d ; Xiaojie Yu^e ; Fanliang Meng^a ; ^d ; Shengqi Wang^b ; ^{sqwang@bmi.ac.cn} ; Jianzhong Zhang^a ; ^d ; ^{zhangjianzhong@icdc.cn}
• 关键词：Staphylococcus aureus ; Quinupristin/dalfopristin ; Resistance ; Livestock-associated ; ST9 ; ABC transporters
• 刊名：International Journal of Medical Microbiology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：306
• 期：8
• 页码：722-729
• 全文大小：1141 K
• 卷排序：306

文摘

Whole-genome sequencing (WGS) was used to investigate the genetic features of the recently identified lsa(E) gene in porcine S. aureus ST9 isolates. Three quinupristin/dalfopristin-resistant isolates harboring the lsa(E) gene (two MRSA and one MSSA) were sequenced. Phylogenetic analysis of 184 S. aureus genomes showed that ST9 porcine isolates belong to a distinct sequence cluster. Further analysis showed that all isolates were deficient in the recently described type IV restriction-modification system and SCCmec type XII was identified in the two MRSA isolates, which included a rare class C2 mec gene complex. A 24 kb ΨSCC fragment was found in the MRSA and MSSA isolates sharing 99% nucleotide sequence homology with the ΨSCCJCSC6690 (O-2) element of a ST9 MRSA isolate from Thailand (accession number AB705453). Comparison of these ST9 isolates with 181 publically available S. aureus genomes identified 24 genes present in all (100%) ST9 isolates, that were absent from the most closely related human isolate. Our analysis suggests that the sequenced quinupristin/dalfopristin-resistant ST9 lineage represent a reservoir of mobile genetic elements associated with resistance and virulence features.