We expressed, for the first time in Pichia pastoris, a set of HEV p179 proteins.
We expressed mutant p179 proteins containing Q, D, P and Y instead of N562.
Asparagine562 is more important for glycosylation than dimerization of p179 protein.
Two p179 mutants were secreted as monomers and elicited HEV neutralizing antibodies.
Neutralizing epitopes presentation and p179 dimerization are not tightly related.